Regulatory

Part:BBa_K753000

Designed by: Rachel Engeler   Group: iGEM12_Chalmers-Gothenburg   (2012-08-29)

Promoter of the FIG1 gene in yeast. The transcription factor Ste12, activated in the yeast pheromone pathway, binds to the FIG1 promoter and initiate the expression of the FIG1 gene.

Five pheromone response elements (PRE, consensus sequence ATGAAACA) have been identified in the 5'-upstream regulatory sequence of the FIG1 gene. Phosphorylated Ste12 binds to them and thereby activates transcription. The PREs in the FIG1 promoter differ slightly from the PRE consensus sequence ATGAAACA:

Sequence Location
TGAAACG -405
TGAAACC* -217
TGACACA* -206
TGAAACG -173
TGAAATA -133
Sites marked with an asterisk occur on the opposite strand

The strictly regulated transcriptional feature of the FIG1 promoter can be useful when investigating the pheromone pathway. The promoter can be used to express reporter genes in response to activation of the pheromone pathway.

Reference: Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated Genes Required for Yeast Mating Differentiation. The Journal of cell biology. 1998;140(3):461-483.

[edit]
Categories
//chassis/eukaryote/yeast
//promoter
//regulation/positive
Parameters
None