Generator

Part:BBa_K737014

Designed by: Tianhe Wang   Group: iGEM12_OUC-China   (2012-09-18)
Revision as of 21:06, 4 May 2014 by Randy (Talk | contribs) (Registry curation)

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gvpC-GFP fusion protein UH

This part composed of fusion gene between gvpC-20 and GFP mut 3.1 by using rigidity hinge which Length can achieve 41 Å and Fad-Fad Angle is 123 °a The fusion protein is used to detect the expression of gvpC within the cell volume.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 70
    Illegal NheI site found at 93
    Illegal NheI site found at 133
    Illegal NheI site found at 156
    Illegal NheI site found at 196
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1381

Circular specification. Registry curation 5-4-2014

This part was defined as a composite part J23106 B0034 Self - B0015. This definition is recursive and not valid. The designer should define a basic part with only the parts sequence and then define a separate composite part with the enclosing parts. The current part is enclosed in many copies of the J23106 RBS and Terminator

We have redefined it as a basic part.


[edit]
Categories
Parameters
None