Part:BBa_K658003:Design

a bacteria population-control device with RBS0.6 driven by lacl+pL

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 718
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2347
  Illegal BsaI.rc site found at 1874
  

Design Notes

The part was designed to combine three parts together to construct a population-control device.The three subparts are a LuxI producer, a LuxR producer and a killer protein producer.

1) LuxI producer

2) LuxR producer

3)killer protein producer

The device is successfully constructed which can be verified by the restriction analysis shown as follows: