Part:BBa_K648006
Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
This is the longest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the ten amino acids GENLYFQSGG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
This linker was used in a very interesting article, entitled “Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion”. The work addressed the use of the linker in question in the construction of genetic circuits. Being represented as a spacer region in the resulting rtTA-GFP fusion protein, obtained by in-frame insertion of the translated region of yEGFP into the KpnI site of rtTA. It has also been cited in a patent relating to a fc Receptor (fcrn) linking peptides and uses thereof. Where it has been described as a simple 10AA long fusion protein linker comprising glycine and serine residues, it can be used for the formation of cyclic dimeric peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Isoelectric Point: 4,00
Molecular Weight: 1071,11Da
References
- Becskei A, Séraphin B, Serrano L. Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion. EMBO J. 2001;20(10):2528-2535.
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