Part:BBa_K638404

polyHis-ReflectinA1

This is our reflectin A1 biobrick with an N-terminal 6-His tag, with the ribosome binding site B0034.

The coding region is for the [http://www.ncbi.nlm.nih.gov/nuccore/FJ824804.1 Reflectin A1 protein], originally found in the mantle of squid Loligo pealeii. It has been codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638202 , and used it to produce brightly coloured responsive thin films. See our [http://2011.igem.org/Team:Cambridge/Project/Microscopy#/Media#Dynamic_Iridescence reflectin thin films] in action or read more about [http://2011.igem.org/Team:Cambridge/Pr polyHis-ReflectinA1

This is our reflectin A1 biobrick with an N-terminal 6-His tag, with the ribosome binding site B0034.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 43
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

oject/Background the background to this protein].

Usage and Biology

In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour from thin film interference effects. In L. pealeii reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour]. Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 43
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]