Tag

Part:BBa_K638402

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-09-19)
Revision as of 14:42, 20 September 2011 by J3harvey (Talk | contribs)

TorA tag variant

This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here].

We ordered this sequence as a synthetic oligonucleotide in two halves: one 5-->3, one 3-->5, with 20bp overlap. We then joined these parts using PCR to make the full sequence. This is essentially a deliberate primer dimer. We then inserted this sequence into our previous constructs using [http://www.synbio.org.uk/gibson/downloads/files/RFC57.pdf Gibson assembly], an extremely powerful method of assembling DNA constructs that is compatible with Standard Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTorA tag variant