Part:BBa_K633000

CelD Mutated Cellulase

Clostridium thermocellum endoglucanase CelD is a representative enzyme for detailed structural and functional studies of the family E cellulases.
Beside C. thermocellum’s CelD, E family cellulases include a number of cellulases of bacterial, fungal, and plant origin.
CelD, has been previously overexpressed in Escherichia coli, the enzyme is easily purified in large amounts from cytoplasmic inclusion bodies. (Chauvaux, Beguin, Aubert, 1992)
The CelD sequence used has an aminoacid substitution Asp-523 ---> Ala. That mutation increases the specific activity of the enzyme by 224% (Chauvaux, Beguin, Aubert, 1992)

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References
Chauvaux, S., Beguin, P., & Aubert, J. (1992). Site-directed mutagenesis of essential carboxylic residues in clostridium thermocellum endoglucanase celd*. The Journal of Biological Chemistry, 267(5), 4472-4478.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1831
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 644
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 508
  Illegal AgeI site found at 151
  Illegal AgeI site found at 567
  Illegal AgeI site found at 1396
  
• 1000
  COMPATIBLE WITH RFC[1000]