Blue Fluorescent Protein with B0032 RBS, this part can be added downstream to any promoter, assembled into pSB3K3 backbone and tested for promoter strength. B0032 is the standard RBS for promoter tests in pSB3K3 backbone.
We hypothesized that the expression level of mTagBFP is affected by the presence or absence of a terminator. To check if that was true, we compared fluorescence outputs from BFP generators with terminator, versus one without a terminator.
The results showed that the observed blue fluorescence outputs were lowered when the transcription was not properly terminated.
In this experiment, we have improved BBa_K592023 (BBa_B0032-BBa_K592100) by adding a terminator BBa_B1006 3’ to this part in hoping to provide future users with better reporters for their assays. We also would like to raise the awareness on the standards of parts measurement through this investigation. We cautioned on the direct use of previous measurement data for promoters generated using BBa_K592023, and we suggest future iGEM teams that undertake measurement experiments of any kind not to omit parts that might appear irrelevant in the measurement cassettes.
*Please refer to BBa_K1899001 as the improved part.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC