Composite

Part:BBa_K575024

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)
Revision as of 19:50, 24 September 2011 by Nirmitd7 (Talk | contribs)

Standardized Fluorescence for S1.jpg

When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control.


LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0030), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830


[edit]
Categories
Parameters
n/aLasP/30/G +CP/30/LasR