Part:BBa_K546546

The Synchroscillator: A Tunable Synchronized Oscillatory System

--Still under construction--

This BioBrick device yields an oscillating pattern of GFP (with an LVA Tag) expression, in the way described below. It contains two differing hybrid promoters that allows for stabilization of the oscillation.

Usage and Biology

The Tunable Syncrhonized Oscillatory System (Synchroscillator) consists mainly of one negative feedback loop, i.e. a biological process that downregulates itself, one positive feedback loop, i.e. a biological process that induces itself, and the reporter protein Green Fluorescent Protein (GFP). Both feedback loops are regulated by hybrid promoters that can decrease the transcription rate upon presence of the Tet Repressor Protein (TetR) or lac repressor (LacI) DNA-binding protein.

The positive feedback loop is created by the quorum sensing (QS) molecule 3OC6HSL. (This QS molecule is able to diffuse through a colony of bacteria and was found in a Vibrio species that uses it to react to its cell density in a culture.) 3OC6HSL is produced by the enzyme encoded by the luxI gene. Most important of this is that 3OC6HSL is able to increase the transcription rate of the luxI gene, in the whole colony, again. It does so, by binding to the hybrid 3OC6HSL-LacI promoter if LacI is absent.

Interacting with the positive feedback loop is the negative feedback loop. Also this feedback loop is caused by presence of the QS molecule.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2781
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1184
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 985
  Illegal BsaI.rc site found at 2042
  Illegal BsaI.rc site found at 2930
  Illegal BsaI.rc site found at 3657