Device
Part:BBa_K546546
Designed by: M. Hesselman and B. Ryback Group: iGEM11_Wageningen_UR (2011-09-14)
The Synchroscillator: A Tunable Synchronized Oscillatory System
This BioBrick device yields an oscillating pattern of GFP (with an LVA Tag) expression. It contains a plux-lac hybrid promoter that enables fine tuning of the oscillation by LacI presence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2781
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1184
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 2042
Illegal BsaI.rc site found at 2930
Illegal BsaI.rc site found at 3657
[edit]
Categories
Parameters
control | (1) J23101 (2) K176000 (3) I751502 (4) R0062 |
device_type | feedback |
direction | Forward |
latency | Seconds |
protein | (1) LuxR _ (2) AiiA-LVA (3) LuxI-LVA (4) GFP-LVA |
signalling_molecule | 3OC6HSL (AHL) |
switch_point | (1) 2nM |