Device

Part:BBa_K546546

Designed by: M. Hesselman and B. Ryback   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 18:38, 16 September 2011 by PimK (Talk | contribs)

The Synchroscillator: A Tunable Synchronized Oscillatory System

This BioBrick device yields an oscillating pattern of GFP (with an LVA Tag) expression. It contains a plux-lac hybrid promoter that enables fine tuning of the oscillation by LacI presence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2781
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2042
    Illegal BsaI.rc site found at 2930
    Illegal BsaI.rc site found at 3657


[edit]
Categories
Parameters
control(1) J23101 (2) K176000 (3) I751502 (4) R0062
device_typefeedback
directionForward
latencySeconds
protein(1) LuxR _ (2) AiiA-LVA (3) LuxI-LVA (4) GFP-LVA
signalling_molecule3OC6HSL (AHL)
switch_point(1) 2nM