Measurement

Part:BBa_K546002:Design

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 18:39, 16 September 2011 by PimK (Talk | contribs) (→‎Design Notes)

Lux pL controlled luxR with lux pR controlled GFP(lva tag).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828


Design Notes

The original R0063 BioBrick part (promoter) of the F2621 BioBrick device (R0063-B0034-C0062-B0010-B0012-R0062) turned out to be absent in this composite BioBrick part, as was shown by a sequence analysis. This K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: K546003.

Source

The lux p promoters originate from the quorum sensing ability of Vibrio fischeri.

References