Measurement

Part:BBa_K516132

Designed by: Susanna Zucca, Federica Sampietro, Giuseppe Bertoni   Group: iGEM11_UNIPV-Pavia   (2011-09-08)
Revision as of 14:46, 23 September 2011 by Fedesampi (Talk | contribs)

Constitutive promoter [J23101] with mRFP, RBS B0032

Protein Generator regulated by J23101 constitutive promoter with B0032 medium efficiency RBS, mRFP Reporter Coding Region and B0015 double Transcription Terminators.
BBa_J23101 is the reference standard promoter for the computation of RPUs.
This measurement device belongs to a set of four parts useful to estimate the RBS efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aConstitutive promoter [J23101] with mRFP, RBS B0032