Measurement
Part:BBa_K516132
Designed by: Susanna Zucca, Federica Sampietro, Giuseppe Bertoni Group: iGEM11_UNIPV-Pavia (2011-09-08)
Constitutive promoter [J23101] with mRFP, RBS B0032
Protein Generator regulated by J23101 constitutive promoter with B0032 medium efficiency RBS, mRFP Reporter Coding Region and B0015 double Transcription Terminators.
BBa_J23101 is the reference standard promoter for the computation of RPUs.
This measurement device belongs to a set of four parts useful to estimate the RBS efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 617
Illegal AgeI site found at 729 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Constitutive promoter [J23101] with mRFP, RBS B0032 |