Coding

Part:BBa_K511205:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)
Revision as of 01:06, 21 October 2011 by Grantrob (Talk | contribs) (→‎Source)

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LacI-KRAB Repressor MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1417
    Illegal SapI.rc site found at 1381


Design Notes

Not applicable.


Source

This protein is a fusion between a mammalian codon-optimized version of the LacI repressor originally derived from Escherichia coli and the Kruppel-associated box derived from a human zinc finger repressor. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References