Coding

Part:BBa_K511202:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 01:05, 21 October 2011 by Grantrob (Talk | contribs) (→‎Source)

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rtTA-DD Transactivator MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 16
    Illegal BsaI.rc site found at 655


Design Notes

Note that the DD domain is positioned before the rtTA transactivator in this part.


Source

This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team.

References