Reporter

Part:BBa_K511101:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 01:02, 21 October 2011 by Grantrob (Talk | contribs) (→‎Source)

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EYFP-FF4x4 MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The presence of FF4 binding sites on this protein will preclude its use in certain cells that naturally produce the FF4 miRNA, and it is recommended that parties interested in using this part consult references explaining which miRNAs are produced in their desired cell types.


Source

This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has ben modified to fluoresce yellow. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References