Conjugation

Part:BBa_K4971011

Designed by: Yi Peng   Group: iGEM23_PIHS-Beijing   (2023-10-12)


Linker protein sequence with starting codon

This is a linker sequence composed of Serine and Glycine amino acids we used to link our starting codon with a hexa-histidine tag. It is used both to separate the his-tag from the starting codon int the restriction enzyme site(we used an NcoI site before our protein), and to make sure the inlaid starting codon in the restriction site does not cause frameshift problems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None