RBS

Part:BBa_K4951006

Designed by: Julia Vu   Group: iGEM23_Stanford   (2023-10-08)


KanR Toehold 7

The toehold switch is an RNA switch designed against the KanR gene using NuPack software, effectively functioning as an RBS and working to control the transcription of the gene it follows. The toehold was designed to control essential gene 2.5 in T7 phages and is preceded by homology arms that correspond to the T7 phage genome, specifically the region in front of gene 2.5. The part also contains a T7 promoter, the designed toehold switch, and followed by a homology arm that corresponds to the sfGFP gene for in vitro cell-free testing purposes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None