RBS

Part:BBa_K4911000

Designed by: Glukhov Grigory   Group: iGEM23_SMBU   (2023-10-11)


Modified sequence of a part BBa_J433006 with modified BsaI 3

This part is a modification BBa_J433006 .This part is required for assembly using the RFC 1000 standard. This part allows you to use proteins from Fluorescent Reporter Proteins Collection, CRISPR-Cas Collection with promoters from Asimov Mammalian Parts Collection. This part is obtained from BBa_J433006 by adding the kozak sequence and BsaI 3' fusion site AATG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 81


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Categories
Parameters
None