RBS

Part:BBa_K4813019

Designed by: Chong Tsz Ting, Jason   Group: iGEM23_HongKong-JSS   (2023-10-08)


Modified RBS for T7 promoter

The RBS in pET28a(+)-FGF2 is modified to increase gene expression and product yield [1]. We use this modified RBS in our construct to enhance the expression of our gene of interest.

References

[1]Shilling, P. J., Mirzadeh, K., Cumming, A. J., Widesheim, M., Köck, Z., & Daley, D. O. (2020). Improved designs for pet expression plasmids increase protein production yield in escherichia coli. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-0939-8

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None