RBS

Part:BBa_K4728015

Designed by: Michelle Li   Group: iGEM23_Queens-Canada   (2023-10-08)


PhaJ (Hydratase)

PhaJ ((R)-Specific enoyl-CoA hydratases) catalyzes the oxidation of enoyl-CoA to (R)-3-hydroxyacyl-CoA. Where (R)-3-Hydroxyacyl-CoA is the substrate for PhaC and the direct precursor of PHA biosynthesis. This occurs if the carbon source is oxidized through the fatty acid b-oxidation pathway (Tsuge et al., 2015). This enzyme converts trans-2-enoyl-CoA into (R)-3-hydroxyacyl-CoA and uses an intermediate originating from the beta oxidation cycle used for the metabolism of fatty acids. (R)-3-hydroxyacyl-CoA monomers can be polymerized into polyhydroxybutyrate (PHB) using PhaC (Sato et al., 2011). This enzyme is involved in the biosynthesis of PHB with an approximate mass of 18kDa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None