Part:BBa_K4706009

SrpR codon optimised S. cerevisae

SrpR is a repressor usually only used in E. coli. This part is codon optimized for S. cerevisae expression to be used in repression systems in a S. cerevisae chassis.

this part does not entail a stop codon as for our usecase we needed to fuse the protein together with a SV40-NLS Tag (see part BBa_K4706021 for more details)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 244
  Illegal BglII site found at 514
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 490
  
• 1000
  COMPATIBLE WITH RFC[1000]