Part:BBa_K4706006

E2A self cleaving peptide

E2A is a Self cleaving peptide these are also commonly called 2A peptides. In the work from Tatiana M Souza-Moreira et al. they screened multiple 2A peptides and compared their cleaving efficiency in Saccharomyces cerevisiae. E2A from all 22 2A peptides tested showed the highest self cleaving efficiency with >90%.

This coding part does not entail a stop codon as it is not meant to be used at the end of a sequence. If you still want to use this self cleaving Peptide at the end of your Protein, remember to add a stop codon!

See also: BBa_K3544407

Usage and Biology

2A self cleaving peptides allow for expression of two or more proteins while being flanked by only one promoter and one terminator which can lead to a reduced pathway construction time. This also opens the possibility of utilizing A2 peptides to express the Protein of interest in conjunction with a reporter (As shown in BBa_K4706013; BBa_K4706015; BBa_K4706016).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References: 1. Tatiana M Souza-Moreira, Clara Navarrete, Xin Chen, Cleslei F Zanelli, Sandro R Valentini, Maysa Furlan, Jens Nielsen, Anastasia Krivoruchko, Screening of 2A peptides for polycistronic gene expression in yeast, FEMS Yeast Research, Volume 18, Issue 5, August 2018, foy036, https://doi.org/10.1093/femsyr/foy036