RBS

Part:BBa_K4666023

Designed by: Jinyu Wang   Group: iGEM23_HUBU-China   (2023-10-04)


RBS-9K

Ribosomal binding sites refer to a purine rich untranslated region upstream of the start codon AUG. Also known as SD (Shine-Dalg-arno) sequence. In RBS, there is a sequence of SD (Shine-Dalg-arno) with a length of 5 nucleotides, which is rich in G and A. The sequence complements with the 3'end of ribosome 16S rRNA, facilitating the binding of ribosomes to the mRNA and facilitating the initiation of translation. The binding strength of RBS depends on the structure of SD sequence and the distance between SD sequence and AUG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None