Part:BBa_K4633007

RBS paraE - the RBS from the promoter for the araE gene from Bacillus subtilis 168

Usage and Biology

The RBS (Ribosome Binding Site) sequence provided here is designed to be used in conjunction with the promoter for the araE gene, L-arabinose transporter, a component of the L-arabinose operon in Bacillus subtilis.

The first 7 bases of this RBS form the core recognition site for ribosome binding (GGGAGGG) [1]. Following these initial bases, there is a spacer sequence to ensure proper positioning of the ribosome and the start codon for translation initiation.

This specific RBS sequence has been tested and characterized in Bacillus subtilis 168, and its functionality has been assessed in the context of the araE promoter (BBa_K4633006).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization and Measurements

The functionality of this RBS sequence was evaluated by measuring its ability to facilitate efficient translation initiation in Bacillus subtilis 168. It was tested in combination with the araE promoter (BBa_K4633006) and mCherry (BBa_J06504) to ensure compatibility and effectiveness in driving protein expression.

References

• [1] Sá-Nogueira, I., Nogueira, T. V., Soares, S. & De Lencastre, H. The Bacillus subtilis L-arabinose (ara) operon: Nucleotide sequence, genetic organization and expression. Microbiology 143, 957–969 (1997).