Part:BBa_K4432013

Synthetic RBS designed for dxs

This part is a synthetic RBS specifically designed for the Escherichia coli 1-deoxyxylulose-5-phosphate synthase (dxs) (BBa_K3166061) using the Salis Lab RBS Calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of dxs (BBa_K3166068) under the control of the T5 promoter (BBa_K4432000) in the composite parts BBa_K4432122, BBa_K4432320 and BBa_K4432321.

Using the Salis Lab RBS Calculator v2.0 [1,2], the predicted features of this RBS are:

Translation Initiation Rate (au)	:	1125127.53
dG_total (kcal/mol)	:	-13.58
dG_mRNA_rRNA (kcal/mol)	:	-18.48
dG_spacing (kcal/mol)	:	0.0
dG_standby (kcal/mol)	:	0.0
dG_start (kcal/mol)	:	-1.19
dG_mRNA (kcal/mol)	:	-6.1
Accuracy (warnings)	:	OK

References

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  Illegal BamHI site found at 8
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]