RBS

Part:BBa_K4392000

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-09-30)


Ribosome binding site associated with the T7 system from the T7 bacteriophage

This part constitutes the ribosomal binding site associated with the T7 system from the T7 bacteriophage. The mRNA transcript of this sequence is used as a site for the ribosome to bind to prior to initiating translation starting from the start codon. This part is to be used in T7 overexpression systems.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 16
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None