Part:BBa_K427009

Intelligent arabinose biosensor with differential response

This part was the final construct of the Tec-Monterrey iGEM Team in 2010. It senses the concentration of arabinose through the PBad family of promoters. Then amplifies it through sensitivity tuners, the activator proteins of the tuners are jammed by reverse arabinose promoters. The sensitivity tuners activate the production of the three fluorescent proteins. This device was created as a proof of concept for the combination of the jammers and sensitivity tuners, rather than using the whole part, the construct design is what should be regarded.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 228
  Illegal NheI site found at 655
  Illegal NheI site found at 1020
  Illegal NheI site found at 1306
  Illegal NheI site found at 1671
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 168
  Illegal BamHI site found at 715
  Illegal BamHI site found at 960
  Illegal BamHI site found at 1366
  Illegal BamHI site found at 1611
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2975
  

References

Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.

Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.

Mathee, K. a. (1990). Identification of a Positive Regulator of the Mu Middle Operon. Journal of bacteriology, 6641-6650.