Part:BBa_K427002

Mor protein of Mu bacteriophage

Mor is a protein of the Mu bacteriophage. It is typically transcribed by the early promoter of the phage and it activates the middle promoter Pm. Since it is the activator of Pm it can be used alongside this promoter to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.

Usage and Biology

According to Kumaraswami (2004) two domains can be observed on the protein a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the protein can only activate the promoters when in dimers.

This part has no special safety considerations.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.