Translational_Unit

Part:BBa_K427001

Designed by: Jan Marte Contreras Ortiz   Group: iGEM10_Tec-Monterrey   (2010-10-22)
Revision as of 10:56, 27 October 2010 by Janmarte (Talk | contribs)

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C protein of the Mu bacteriophage

C is a protein of the Mu bacteriophage. It is typically transcribed by the middle promoter of the phage (Pm) and it activates the four late promoters Plys, Pi, Pp and Pmom. Since it is the activator of the late it can be used alongside them to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.

Usage and Biology

According to Kumaraswami (2004) two domains can be observed on the protein a Helix-Turn-Helix (HTH) domain and a dimerization domain, which suggests that the protein can only activate the promoters when in dimers.

This part has no special safety considerations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.

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Parameters
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