DNA

Part:BBa_K416002

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-06-01)

36 Base Pair LoxP

The loxP site is the substrate of the Cre recombinase. In nature the site is a palidromic 34bp sequence with a directional 8bp core. Because the site is not a multiple of 3, if it is used within coding regions it will throw the remainder of the sequence out of frame. We modified the loxP sequence by adding 2 bases to the 3' end. The resulting biobrick can now be used within transcribed assemblies without modifying the frame or the surrounding biobricks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/cre
Parameters
None