RBS

Part:BBa_K4156081

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-06)


RBS spoVG

SpoVG is an endogenous ribosome binding site (RBS).

Usage and Biology

In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit ( BBa_K4156101 )

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None