Coding

Part:BBa_K4016000

Designed by: Zhixin Fang   Group: iGEM21_NUDT_CHINA   (2021-10-01)
Revision as of 02:35, 15 October 2021 by Qiuxinyuan12 (Talk | contribs)


PixE

PixE is a PatA-like two-component response regulator that interacts with PixDin vivo. It control the phototaxis in the cyanobacterium Synechocystis sp

Usage and Biology

PixE (Slr1693) is a PatA-like two-component response regulator that interacts with PixD in vivo. PixD and PixE can associate in the dark into large multi-subunit complexes that dissociate into dimers of PixD and monomers of PixE within seconds upon blue light stimulation. Upon a shift back to darkness, PixD cycles back to its binding-competent state within seconds to re-form complexes. In our project, this part was used to build light-inducible targeting module for the Trim21, in order to make target protein degradation mediated by PixE/PixD interaction in blue light/dark.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None