RBS

Part:BBa_K3985023

Designed by: Andrea Aquize, Martina Buerkle, Maira Bradler, Sidney Mitsch, Emilia Marie Wrede   Group: iGEM21_TU_Darmstadt   (2021-10-16)


RBS strong for B. subtilis

This strong RBS was engineered by Guiziou et al. (2016) resulting in high expression of sfGFP in B. subtilis.[1]

We used this RBS in our kill-switch cassette for B. subtilis since it was proven to lead to high expression of sfGFP and mKate2 in combination with the Pveg promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None