Conjugation

Part:BBa_K3946045

Designed by: Ritupreet Kaur   Group: iGEM21_Edinburgh   (2021-10-21)


Sb7 silica tag (C)-LCC-WCCG variant

A short silica-binding tag to add to the C-terminal of the LCC-WCCG gene using JUMP assembly. The tag facilitates immobilisation to silica surfaces, enabling enzyme immobilisation or purification using silica-based spin columns.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
    Illegal NheI site found at 227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None