Conjugation

Part:BBa_K3946044

Designed by: Ritupreet Kaur   Group: iGEM21_Edinburgh   (2021-10-21)


Sb7 silica tag (C)-PETase (W159H/ S238F)

A short silica-binding tag to add to the C-terminal of the double mutant PETase gene using JUMP assembly. The tag facilitates immobilisation to silica surfaces, enabling enzyme immobilisation or purification using silica-based spin columns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 659
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None