Conjugation

Part:BBa_K3946007

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-21)


L2NC+linker+term (CT)

Part BBa_K3946003 with terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 541
    Illegal suffix found in sequence at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 541
    Illegal NheI site found at 459
    Illegal NheI site found at 482
    Illegal SpeI site found at 750
    Illegal PstI site found at 764
    Illegal NotI site found at 547
    Illegal NotI site found at 757
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 541
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 541
    Illegal suffix found in sequence at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 541
    Illegal XbaI site found at 556
    Illegal SpeI site found at 750
    Illegal PstI site found at 764
    Illegal NgoMIV site found at 2382
    Illegal NgoMIV site found at 2511
    Illegal NgoMIV site found at 3005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 577
    Illegal BsaI.rc site found at 730
    Illegal SapI.rc site found at 3803


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Categories
Parameters
None