Composite

Part:BBa_K3900063

Designed by: Lucas Krause   Group: iGEM21_Bielefeld-CeBiTec   (2021-10-01)


T7p:10xHis:mRuby3:T7t

Composition for protein expression, mRuby3 in our case, in bacteria strains possessing the T7 Polymerase and subsequent His-tag purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 760
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 65


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Categories
Parameters
None