RBS

Part:BBa_K3885113

Designed by: Xue Li   Group: iGEM21_ZJUT-China   (2021-10-10)


UTR3

UTR1 (BBa_K3885111) with mutation in RBS.

Characterization

The UTR1 mutation to UTR3 was used to construct P70a.b.c-UTR3

Figure 1. Comparing expression in TXTL reactions to the model. Maximum rate of deGFP synthesis expressed from varying concentrations of P70a-UTR3-deGFP(BBa_K3885313), P70b-UTR3-deGFP (BBa_K3885323), P70c-UTR3-deGFP (BBa_K3885333)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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