Part:BBa_K382002

pORE Expression Series Vector with BioBrick MCS, Basta resistance

Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the pENTCUP2 promoter upstream of MCS for constitutive expression of gene of interest.

We used this vector for integration of several gene constructs into Arabidopsis.[http://2010.igem.org/Team:Harvard/results]

Plant transformed with this vector:

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 5326
  Illegal XbaI site found at 5299
  Illegal SpeI site found at 5378
  Illegal PstI site found at 5356
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5326
  Illegal NheI site found at 4766
  Illegal SpeI site found at 5378
  Illegal PstI site found at 5356
  Illegal NotI site found at 5341
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5326
  Illegal BglII site found at 5212
  Illegal BglII site found at 7771
  Illegal BamHI site found at 5312
  Illegal XhoI site found at 4790
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 5326
  Illegal XbaI site found at 5299
  Illegal SpeI site found at 5378
  Illegal PstI site found at 5356
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 5326
  Illegal XbaI site found at 5299
  Illegal SpeI site found at 5378
  Illegal PstI site found at 5356
  Illegal NgoMIV site found at 625
  Illegal NgoMIV site found at 749
  Illegal NgoMIV site found at 2181
  Illegal NgoMIV site found at 2772
  Illegal NgoMIV site found at 7220
  Illegal AgeI site found at 341
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 7241