Composite

Part:BBa_K3796207

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)
Revision as of 02:33, 21 October 2021 by Yang Feiyue (Talk | contribs)


Patp2-rbs-gfp-terminator

This part is composed of an inducible promoter Patp2, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3796213). P-atp2 is an alkali inducible promoter found in Corynebacterium glutamicum. Under high pH, the gfp will have higher expression, leading to stronger fluorescence intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None