Composite
Part:BBa_K3796207
Designed by: Yang Feiyue Group: iGEM21_CAU_China (2021-09-24)
Revision as of 02:33, 21 October 2021 by Yang Feiyue (Talk | contribs)
Patp2-rbs-gfp-terminator
This part is composed of an inducible promoter Patp2, a rbs sequence in Corynebacterium glutamicum (BBa_K2963006), the fluorescent protein gene bjGFP, and the rrnB T1 and T2 terminators (BBa_K3796213). P-atp2 is an alkali inducible promoter found in Corynebacterium glutamicum. Under high pH, the gfp will have higher expression, leading to stronger fluorescence intensity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 14
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 14
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 14
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 14
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |