Part:BBa_K3718002
Customizable detection and expression with increased growth
Part II of the Toolkit for Detection with Proliferation Control
The Modular Receptor Platform (MRP) is designed according to the paper published in 2018 (Chang et al, 2018). When the antigen/ligand binds to the nanobody, dimerization of the protein CadC domains occur. As a result, this allows the binding of the pCadBA operon region and transcription downstream is promoted at the same time. The VHH region has high flexibility as it can be customized for binding with different targets, so that any specific targets with single-domain antibodies available will be able to bind with the VHH region for detection. Users can add their own VHH region after the transmembrane region DNA via restriction ligation.
For the developers to clone the gene(s) that they want to express, a multiple cloning site (MCS) K660500 to allow for the insertion of the target genes. arcA and iclR genes are originally knocked out using Part I of our toolkit to reduce growth. When the antigen is detected, arcA and iclR are expressed to restore growth while protein(s) of choice are expressed.
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Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 67
Illegal suffix found in sequence at 629 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 67
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 630
Illegal PstI site found at 644
Illegal NotI site found at 73
Illegal NotI site found at 637 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 67
Illegal BglII site found at 2337
Illegal BamHI site found at 912
Illegal XhoI site found at 906 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 67
Illegal suffix found in sequence at 630 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 67
Illegal XbaI site found at 82
Illegal SpeI site found at 630
Illegal PstI site found at 644 - 1000COMPATIBLE WITH RFC[1000]
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