RBS
Part:BBa_K3601004
Designed by: Daniel Castellano Garrido Group: iGEM20_UCL (2020-10-21)
Strong RBS
This part is a strong RBS from the parental vector pET3a to be used in E. coli BL21 cells for efficient protein translation. This was used to express PETase-MHETase and MHETase-PETase fusion proteins as well as PETase and MHETase on its own.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |