RBS

Part:BBa_K3601004

Designed by: Daniel Castellano Garrido   Group: iGEM20_UCL   (2020-10-21)


Strong RBS

This part is a strong RBS from the parental vector pET3a to be used in E. coli BL21 cells for efficient protein translation. This was used to express PETase-MHETase and MHETase-PETase fusion proteins as well as PETase and MHETase on its own.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None