Part:BBa_K3598010
Human CathelicidinLL-37
Human Cathelicidin LL-37 is antimicrobial peptides produced by human in response to various pathogenic microbes. It has been shown to possess potent antimicrobial activity against a variety of bacteria.
Usage and Biology
The Human cathelicidin LL-37 coding part was the original sequence of Human cathelicidin LL-37 we sent to a synthetic company to produce our Human cathelicidin LL-37 AMP. We verified the antimicrobial potency of Human cathelicidin LL-37 purchased from the company by adding its solution and placing pieces of soaked with its solution to plates inoculated with P. acnes and E. coli MG1655. The results showed inhibition zones in areas of direct contact between bacteria and Human cathelicidin LL-37, which means Human cathelicidin LL-37 is effective in killing both bacteria, and would not affect bacteria outside its contact.
Then, we verified Human cathelicidin LL-37's ability to kill E. coli MG1655 and P. acnes by adding its solution to liquid culture inoculated with the two bacteria. We set the AMP concentration gradient as 0, 2, 4, 8, 12, 16 mg/L, and measured the OD600 of the E. coli through time. Since P. acnes has a slow growth rate, we only measured OD600 after 24 hours of cultivation. The results indicate that Snake Cathelicidin-BF's ability to kill E. coli MG1655 peaked at concentration 4mg/L at 8h, its effect reaching an OD600 of 2.0. Human cathelicidin LL-37's ability to kill P. acnes peaked at concentration 16mg/L, its effect reaching an OD600 of about 0.15.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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