Coding

Part:BBa_K3544004:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)
Revision as of 13:17, 27 October 2020 by SCU-XYL (Talk | contribs) (→‎Design Notes)


Csy4,Encoding endonuclease that specifically recognizes and cleaves 28nt stem-loop structure


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 148
    Illegal XbaI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 27


Design Notes

Csy4 can specifically cleave the 28nt stem-loop structure to separate the target fragments before and after the stem-loop structure

Source

Pseudomonas aeruginosa

References