Composite
PBad LuxI

Part:BBa_K3484005

Designed by: Tomas Berjaga, Quim Marti & Jaume Puig   Group: iGEM20_UPF_Barcelona   (2020-10-26)
Revision as of 16:23, 26 October 2020 by Tomasberjaga (Talk | contribs)


Bad promoter + LuxI

This construct synthesizes lactone via the LuxI enzyme. This enzyme is regulated by the pBad promoter, which is induced by Arabinose but repressed by Glucose. This means that the production of lactone can be controlled in two ways, increasing the production with Arabinose and decreasing it with Glucose.

A schematic representation of the interactions is shown below (Fig.1). Notice that when LuxI is formed, this enzyme produces lactone (AHL).

Figure 1. Scheme of the interactions on the Pbad LuxI composite part.

This system allows to have a control of the AHL lactone production in a really consistent way, and consequently, is a system that could be used to simulate other different ones. The composite part is Biobrick compatible and was characterized in E.Coli DH5α.

This composite part is composed by all the following parts:

BBa_B0015: A reliable double terminator that consists on BBa_B0010 and BBa_B0012.

BBa_B0032: A medium RBS. The RBS.3 (medium) (derivative of BBa_0030

BBa_I13453: PBad promoter from I0500 without AraC

BBa_C0061: LuxI gene


Model & Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 931
    Illegal BamHI site found at 202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None