Composite
AHL sfGFP
Part:BBa_K3484004
Designed by: Tomas Berjaga, Quim Marti & Jaume Puig Group: iGEM20_UPF_Barcelona (2020-10-26)
Revision as of 11:11, 26 October 2020 by Tomasberjaga (Talk | contribs)
AHL sfGFP+ASV reporter
This composite part produces sfGFP when a large enough concentration of lactone is present in the medium. In this case,vit constitutively produces LuxR, which binds to the lactone present in the medium and together, they form a complex that activates the pLux promoter, generating the sfGFP whose fluorescence can be measured. The schematics of this composite part can be seen in Figure 1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1249
Illegal AgeI site found at 1372 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1142
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Categories
Parameters
None |