Composite
AHL sfGFP

Part:BBa_K3484004

Designed by: Tomas Berjaga, Quim Marti & Jaume Puig   Group: iGEM20_UPF_Barcelona   (2020-10-26)
Revision as of 11:11, 26 October 2020 by Tomasberjaga (Talk | contribs)


AHL sfGFP+ASV reporter

This composite part produces sfGFP when a large enough concentration of lactone is present in the medium. In this case,vit constitutively produces LuxR, which binds to the lactone present in the medium and together, they form a complex that activates the pLux promoter, generating the sfGFP whose fluorescence can be measured. The schematics of this composite part can be seen in Figure 1.

Figure 1. Scheme of the interactions on the AHL sfGFP+ASV tag reporter sensor cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1142


[edit]
Categories
Parameters
None