Composite

Part:BBa_K3457058

Designed by: Yixian Yang   Group: iGEM20_QHFZ-China   (2020-10-04)
Revision as of 03:18, 27 October 2020 by Leaves (Talk | contribs)

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UA sensor-dsRed-J23100-CAHS 106094

    This biological part is designed to sense uric acid and express CAHS 106094 at the same time. CAHS 106094 is used to protect the bacteria during storage.

Contribution

Group: QHFZ-China iGEM 2020

Author: Yixian Yang

The part is a combination of BBa_K3007029 PhucR-dsRed-Pcp6-HucR & YgfU (uric acid sensor) and BBa_K3457046 J23100-Olac-RBS-CAHS 106094. The design and reslt are as below. However, there is something wrong. The constitutive expression of the reporter, dsRed, was over high. The possible reason is: (1) the construction influence the CP6 promoter, so that the expression of the repressor, HucR, was insufficient; (2) CAHS 106094 affected the uric acid sensor. Further study should be taken.

Figure 1. Design and result.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3263
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal EcoRI site found at 3263
    Illegal NheI site found at 1085
    Illegal NheI site found at 3291
    Illegal NheI site found at 3314
    Illegal NheI site found at 4225
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
    Illegal NotI site found at 3269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal EcoRI site found at 3263
    Illegal BglII site found at 852
    Illegal BglII site found at 3256
    Illegal BamHI site found at 158
    Illegal BamHI site found at 2266
    Illegal BamHI site found at 3130
    Illegal BamHI site found at 3205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3263
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3263
    Illegal EcoRI site found at 1399
    Illegal EcoRI site found at 3216
    Illegal XbaI site found at 3113
    Illegal XbaI site found at 3278
    Illegal SpeI site found at 917
    Illegal PstI site found at 140
    Illegal PstI site found at 510
    Illegal PstI site found at 3210
    Illegal AgeI site found at 2084
  • 1000
    COMPATIBLE WITH RFC[1000]


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