Part:BBa_K3447105
_NOTOC__ The expression of AHL
This is a part that can produce AHL constitutively. LuxI protein is a kind of AHL synthases.
LuxI gene is an important gene for gram-negative bacteria to conduct quorum sensing communication. It is responsible for the synthesis of AHL signaling molecules. AHL, an acyl hyperserine lactone, is the main signal molecule for gram-negative bacteria to communicate quorum sensing information. E. coli is a typical Gram-negative bacterium, but its quoral-sensing system 1 is not complete. It lacks the luxI gene, so it cannot synthesize AHL synthase and thus AHL signal molecules. We hope to introduce the luxI gene into Escherichia coli to produce AHL signaling molecules. (AHL is a class of signaling molecules in which the luxI gene differs from the luxR gene in different species of bacteria. The binding of signal molecules to receptor proteins is species-specific which can prevent interference from other species. However, E. coli is an exception that can sense all kinds of AHL signaling molecules.)
Design
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in GenBank.
References
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |