Coding

Part:BBa_K3429012

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-10-16)
Revision as of 13:10, 26 October 2020 by Phigem (Talk | contribs)


Blue copper oxidase CueO

Profile

Name Copper efflux oxidase CueO
Base pairs 1551
Molecular weight 56.58 kDa
Origin Escherichia coli, synthetic
Properties Copper efflux oxidase CueO from Eschericha coli is a copper dependant bacterial laccase possesing five coordinated copper ions relevant for electron transfer. The Cu-ions are coordinated as a T1, three T2/3-cluster Cus and a fifth weakly coordinated copper. CueO differs from most other laccases in its fifth copper binding site[1].

This part was designed for in vitro characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

[101] L. Zhang et al. Directed Evolution of a Bacterial Laccase (CueO) for Enzymatic Biofuel Cells. Angewandte Chemie 2019, 58 (14) 4562-4565, https://doi.org/10.1002/anie.201814069


[edit]
Categories
Parameters
None